Plasmid_Backbone

Part:BBa_K1137003

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-10-04)

pSB9S1 – Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 – 40 per cell. The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.

PB_PSB9S1.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1929
    Illegal suffix found at 3020
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1929
    Illegal NheI site found at 1005
    Illegal SpeI site found at 3021
    Illegal PstI site found at 3035
    Illegal NotI site found at 1935
    Illegal NotI site found at 3028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1929
    Illegal XhoI site found at 1805
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1929
    Illegal suffix found at 3021
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1929
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1944
    Illegal SpeI site found at 3021
    Illegal PstI site found at 3035
    Illegal NgoMIV site found at 258
    Illegal AgeI site found at 2731
    Illegal AgeI site found at 2843
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None